Isolation, culturing and 3D bioprinting equine myoblasts

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration in vitro meat production. This research describes a protocol to isolate primary from skeletal muscle of an adult horse. The equine expressed markers specific had multipotent potential capabilities with differentiation into chondrocytes, adipocytes osteoblasts vitro. horse did not adhere Cytodex 3 grew poorly on CultiSpher-S microcarriers during cultivation. Our studies showed that the use GelMa bioink ionic cross-linking have negative effects cell proliferation at beginning However, cells reduced proliferative activity by day 40 following culturing. population obtained individual, propagated bioink, meet requirements regenerative veterinary manufacturing regarding quantity quality required. Nonetheless, further optimization scaling up process, including both and/or bioreactor program bioprinting, still important.